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Analysis of the Effect of Parathyroid Hormone (PTH) Treatment on Osteoblastogensis from Leptin Receptor-Positive Mesenchymal Stem Cells
https://mdu.repo.nii.ac.jp/records/2839
https://mdu.repo.nii.ac.jp/records/2839095f781f-cb26-42a2-81de-38815b7d2ebc
| 名前 / ファイル | ライセンス | アクション |
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内容の要旨および審査の結果の要旨 (532.3 kB)
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本文 (3.0 MB)
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| Item type | 学位論文 / Thesis or Dissertation正(1) | |||||
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| 公開日 | 2019-07-23 | |||||
| タイトル | ||||||
| タイトル | Analysis of the Effect of Parathyroid Hormone (PTH) Treatment on Osteoblastogensis from Leptin Receptor-Positive Mesenchymal Stem Cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | 甲状腺ホルモンによるLeptin受容副体陽性間葉系幹細胞の骨芽細胞分化機構の解析 | |||||
| 著者 |
Yang, Mengyu
× Yang, Mengyu |
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| 著者別名 |
楊, 孟雨
× 楊, 孟雨 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Bone marrow mesenchymal stem and progenitor cells (BM-MSPCs) maintain homeostasis of bone tissue by providing osteoblasts. Although studies have suggested that MSPCs are observed as leptin receptor (LepR)-positive cells in BM, these cells still contain non-MSPC populations. On the other hand, genetic lineage tracing approaches revealed that intermittent parathyroid hormone (iPTH) treatment induced osteoblastic differentiation from BM-MSPCs. However, little is known about the mechanistic details of these process in vivo. Here, we demonstrate that the LepR+MSPC population contains Runx2-GFPlow sub-population, which possesses higher stem cell activity, than the Runx2-GFP− stromal population. In response to iPTH treatment, LepR+MSPCs differentiate into Type I collagen a (Col1)+ mature osteoblasts. Along with osteoblastogenesis, the number of Col1+ mature osteoblasts increased around the bone surface, although most were characterized as quiescent cells. However, the number of LepR+MSPCs also increased in the vicinity of bone tissue, and the cell cycle was accelerated in these cells by iPTH treatment. The expression levels of osteoblastic markers were increased in the LepR+MSPC population in response to iPTH treatment. In contrast, the expression levels of markers for adipocytes decreased in this population. Altogether, our results indicate that Runx2 is weakly expressed in the LepR+MSPC population without osteoblastic commitment, and iPTH treatment expanded the LepR+MSPC population and skewed their lineage differentiation from adipocytes toward osteoblasts through cell cycle withdrawal. | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 学位授与年度 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2018 | |||||
| 報告番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 甲第211号 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲第211号 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2019-02-07 | |||||
| 学位名 | ||||||
| 学位名 | 博士（歯学） | |||||
| 学位授与大学 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 33602 | |||||
| 学位授与機関名 | 松本歯科大学 | |||||
