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Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering
https://mdu.repo.nii.ac.jp/records/2976
https://mdu.repo.nii.ac.jp/records/2976bab00dc2-500c-4a4e-b7a3-e7bbb0ba7a18
| 名前 / ファイル | ライセンス | アクション |
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内容の要旨および審査の結果の要旨 (2.5 MB)
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本文 (1.4 MB)
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| Item type | 学位論文 / Thesis or Dissertation正(1) | |||||||
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| 公開日 | 2022-01-06 | |||||||
| タイトル | ||||||||
| タイトル | Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Cryopreservation | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | spontaneous spheroid | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | osteogenic differentiation capability | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | compact bone-derived mesenchymal stromal cells, | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | MSCs | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| その他（別言語等）のタイトル | ||||||||
| その他のタイトル | 骨再生治療を目的とした皮質骨由来間葉系間質細胞によるスフェロイドの凍結保存 | |||||||
| 著者 |
董, 宏偉
× 董, 宏偉
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| 著者別名 |
Dong, Hongwei
× Dong, Hongwei
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Spontaneously formed spheroids from mouse compact bone-derived mesenchymal stromal cells (CB-MSCs) possess enhanced stemness and superior plasticity. In this study,the effect of cryopreservation on viabil ity,stemness,and osteogenic differentiation capability of spontaneous CB-MSC spheroids were investigated.CB-MSCs were isolated from mouse femur and tibia. Spheroids were cryopreserved with various concentra tions of dimethyl sulfoxide (DMSO). After thawing,the number of living and dead cells was measured.The expression levels of stem cell markers and osteogenic marker genes were analyzed. The cryopreserved and noncryopreserved spheroids were transplanted in mice with a beta-tricalcium phosphate as a scaffold to evaluate the in vivo bone-forming capability.The percentage of living cells was highest when 5% DMSO was used as a cryoprotectant,confirmed by the number of dead cells.The expression of stem cell marker genes and osteogenic differentiation capability were maintained after cryopreservation with 5% DMSO. The cryopre served spontaneous CB-MSC spheroids showed remarkable new bone formation in vivo,identical to that of the noncryopreserved spheroids even without osteogenic induction. The cryopreserved spontaneous CB-MSC spheroids retained stemness and osteogenic differentiation capability and highlight the utility of spontaneous CB-MSC spheroids as ready-to-use tissue-engineered products for bone tissue engineering. | |||||||
| 書誌情報 |
en : Tissue Engineering Part C:Methods 巻 27, 号 4, p. 253-263, 発行日 2021 |
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| 情報源 | ||||||||
| 関連名称 | 雑誌掲載論文(電子版） | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 1937-3384 | |||||||
| 権利 | ||||||||
| 権利情報 | ©️ Hongwei Dong et al.2021; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and re- production in any medium, provided the original work is properly cited. | |||||||
| 関連サイト | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.1089/ten.tec.2021.0001 | |||||||
| 関連名称 | Mary Ann Liebert, Inc., publishers | |||||||
| 関連サイト | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064946/ | |||||||
| 関連名称 | PMC | |||||||
| PubMed番号 | ||||||||
| 識別子タイプ | PMID | |||||||
| 関連識別子 | 33267737 | |||||||
| フォーマット | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | application/pdf | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 学位授与年度 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 2021 | |||||||
| 報告番号 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 甲第239号 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第239号 | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2021-07-14 | |||||||
| 学位名 | ||||||||
| 学位名 | 博士（歯学） | |||||||
| 学位授与大学 | ||||||||
| 学位授与機関名 | 松本歯科大学 | |||||||
