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Marmur変法による株化腫瘍細胞DNAの抽出
https://mdu.repo.nii.ac.jp/records/235
https://mdu.repo.nii.ac.jp/records/23599bce50f-bdb8-4e7e-95b9-98194cc4aeed
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
matsumoto_shigaku_05-02-11.pdf (267.8 kB)
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| Item type | Journal Article(1) | |||||||
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| 公開日 | 2012-05-23 | |||||||
| タイトル | ||||||||
| タイトル | Marmur変法による株化腫瘍細胞DNAの抽出 | |||||||
| 言語 | ||||||||
| 言語 | jpn | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| その他（別言語等）のタイトル | ||||||||
| その他のタイトル | DNA Extraction from the Cultured Tumor Cells, and its Properties | |||||||
| 著者 |
菱田, 市和
× 菱田, 市和
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | The present study was aimed, firstly to apply the modified Marmur's method (by Hill) in extracting DNA from the cultured tumor cells, and secondly to investigate some properties of the extracted DNA. The cultured tumor cells had been taken from the rhabdomyosarcoma in rats induced by injecting DMBA, as reported before, and been kept in the Department of Oral Surgery II, Matsumoto Dental College. Five samples were taken from the 70th to 80th generation of the cultured cells. The DNA was extracted by employing the modified Marmur's method of Hill, which is excellent in obtaining the pure DNA. The extracted DNA was examined by the ultra-violet spectrograph, using the Hitachi Spectrophotometer Model 101, and weight measurements in the Burton's method. The ratio of the ultra-violet absorption at the 260nm wave length to that at 280nm varied little, with an average of 1.9, which coincided with the studies reported by other investigators. The mean weight measure was 100μg/ml, with slight deviation. These results might indicate that the modified Marmur's method of Hill is excellent for obtaining the pure DNA from the cultured tumor cells of rats. | |||||||
| 書誌情報 |
松本歯学 巻 5, 号 2, p. 208-212, 発行日 1979-12-31 |
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| 出版者 | ||||||||
| 出版者 | 松本歯科大学学会 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0385-1613 | |||||||
| 書誌レコードID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AN00232590 | |||||||
| フォーマット | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | application/pdf | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
