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Cytochemical Examination of the AMP-PNP-Hydrolyzing Enzymatic Activity in Rabbit Taste Bud Cells : Effects of Inhibitors and Activators of ATP Pyrophosphatase and of Adenylyl Cyclase
https://mdu.repo.nii.ac.jp/records/656
https://mdu.repo.nii.ac.jp/records/6563d41ca61-df7c-4a77-94d2-b0afda5cff4c
名前 / ファイル | ライセンス | アクション |
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Item type | Journal Article(1) | |||||||
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公開日 | 2012-05-24 | |||||||
タイトル | ||||||||
タイトル | Cytochemical Examination of the AMP-PNP-Hydrolyzing Enzymatic Activity in Rabbit Taste Bud Cells : Effects of Inhibitors and Activators of ATP Pyrophosphatase and of Adenylyl Cyclase | |||||||
言語 | en | |||||||
言語 | ||||||||
言語 | eng | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | taste bud | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | 5'-adenylylimidodiphosphate | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | ATP pyrophosphatase | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | rabbit | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | cytochemistry | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
資源タイプ | journal article | |||||||
その他(別言語等)のタイトル | ||||||||
その他のタイトル | ウサギ味蕾細胞におけるAMP-PNP加水分解酵素の細胞化学的研究 : ATPピロホスファターゼおよびアデニル酸シクラーゼの抑制物質・賦活物質の効果 | |||||||
著者 |
ASANUMA, NAOKAZU
× ASANUMA, NAOKAZU
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抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | In the apical regions of rabbit taste bud cells, especially on the surface of the microvilli, there exists an enzymatic activity that hydrolyzes an ATP analogue, 5'-adenylylimi-dodiphosphate. Since our previous study had suggested that this activity was that of either ATP pyrophosphatase or adenylyl cyclase, the effects of inhibitors and activators of these enzymes on the enzymatic activity were examined cytochemically. ATP pyrophosphatase inhibitors (dithiothreitol, sodium fluoride and amiloride) reduced the enzymatic activity, while adenylyl cyclase inhibitors (p-chloromercuriphenylsulfonic acid and 5,5'-dithio-bis (2-nitrobenzoic acid)) did not. The effect of a mild activator of ATP pyrophosphatase (sodium azide) was not generally clear. Forskolin, a potent activator of adenylyl cyclase, did not show any enhancing effect. Ca^<2+> enhanced the enzymatic activity. The results indicate that the enzymatic activity is that ofATP pyrophosphatase and probably that of Ca^<2+>-dependent type. | |||||||
書誌情報 |
松本歯学 巻 27, 号 1, p. 10-20, 発行日 2001-04-30 |
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出版者 | ||||||||
出版者 | 松本歯科大学学会 | |||||||
ISSN | ||||||||
収録物識別子タイプ | ISSN | |||||||
収録物識別子 | 0385-1613 | |||||||
書誌レコードID | ||||||||
収録物識別子タイプ | NCID | |||||||
収録物識別子 | AN00232590 | |||||||
フォーマット | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | application/pdf | |||||||
著者版フラグ | ||||||||
出版タイプ | VoR | |||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |